study on imipenem resistance and prevalence of blavim1 and blavim2 metallo-beta lactamases among clinical isolates of pseudomonas aeruginosa from mashhad, northeast of iran
نویسندگان
چکیده
background and objectives : the main cause of serious nosocomial infections is a gram-negative pathogen known as pseudomonas aeruginosa (p. aeruginosa). carbapenems are widely used as an appropriate treatment for these infections, however resistance to these agents has been observed and is increasing. metallo beta-lactamase (mbls) enzyme is one of the main causes of resistance to carbapenem. in the current study the frequency and production of vim1 and vim2 by imipenem-resistant p. aeruginosa isolates of patients hospitalized in imam reza hospital were evaluated. materials and methods : in this study, 131 clinical samples were collected from patients hospitalized in imam reza hospital in mashhad during a 15-month period from may 2011 to november 2012. after verification of p. aeruginosa isolates, antibiotic resistance patterns of isolates were determined for 14 antibiotics by kirby-bauer standard disk diffusion according to the clsi guidelines. combined-disk test was used for phenotypic determination of mbls-producing isolates and after dna extraction, genotypic determination of vim1 and vim2 metallo beta-lactamase genes was carried out using multiplex- pcr. results : of 63 imipenem-resistant isolates (48.5%), 56 (88.8%) were mbl-producing in phenotypic assessments. also amongst imipenem-resistant isolates, the frequency of vim1 and vim2 genes were 58.7 and 3.17%, respectively. conclusion : the results of the current study along with the results of the other conducted studies in iran in recent years demonstrate that the average resistance to imipenem in p. aeruginosa isolates was 51.3% which has increased in comparison with the results in 2006 (32.9%). it was also determined that the frequency of vim1 gene was more than vim2 gene. in phenotypic assessment by using cd method, 49.6% of isolates were determined as mbls-producing. the sensitivity and specificity of this method were verified in comparison with the results of pcr test.
منابع مشابه
Study on imipenem resistance and prevalence of blaVIM1 and blaVIM2 metallo-beta lactamases among clinical isolates of Pseudomonas aeruginosa from Mashhad, Northeast of Iran
BACKGROUND AND OBJECTIVES The main cause of serious nosocomial infections is a Gram-negative pathogen known as Pseudomonas aeruginosa (P. aeruginosa). Carbapenems are widely used as an appropriate treatment for these infections, however resistance to these agents has been observed and is increasing. Metallo beta-lactamase (MBLs) enzyme is one of the main causes of resistance to carbapenem. In t...
متن کاملPrevalence of IMP and VIM Metallo-Beta-Lactamases in Pseudomonas aeruginosa Isolates from Clinical and Environmental Specimens in Intensive Care Units (ICUs) of Rasht Hospitals, Iran
Introduction: Pseudomonas aeruginosa is one of the leading causes of nosocomial infections, and antibiotic resistance of this pathogen is an important concern in treating such infections. The current work was conducted to investigate the prevalence of bla-IMP, and bla-VIM metallo-beta-lactamase (MBL) among clinical and environmental P. aeruginosa isolates obtained from ICUs of different hospita...
متن کاملIdentification and Characterization of Metallo-β-Lactamases Producing Pseudomonas aeruginosa Clinical Isolates in University Hospital from Zanjan Province, Iran
Background: Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. Methods: A total of 70 P. aeruginosa isolates were identified from patients ...
متن کاملDetection of Ampc and Extended-Spectrum Beta-Lactamases in Clinical Isolates of Pseudomonas Aeruginosa from Patients with Cystic Fibrosis
ABSTRACT Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of...
متن کاملDetection of Metallo-Beta Lactamases Among Carbapenem-Resistant Pseudomonas aeruginosa
BACKGROUND Carbapenems are important drugs used for the treatment of Pseudomonas aeruginosa infections, however metallo-β-lactamases (MBL) are able to efficiently hydrolyze these classes of drugs. Immediate detection of the MBL-producing P. aeruginosa is necessary in order to accurately treat infections caused by this organism. OBJECTIVES To determine the prevalence of MBL producing P. aerugi...
متن کاملDetection of Different Types of Metallo-β-Lactamases among Pseudomonas aeruginosa Isolates Obtained from Intensive Care Unit Patients
Introduction: Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy, due to chromosomal mutations or intrinsic resistance to various antimicrobial agents, such as Metallo-β-Lactams (MBL). This study aimed to investigate the prevalence of β-lactamases encoding genes among P. aeruginosa strains isolated from intensive care unit (ICU) patients by phenotypic and multiplex PCR meth...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of microbiologyجلد ۷، شماره ۲، صفحات ۷۲-۷۸
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023